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Smart Lab publishes first detailed analysis of gene expression inheritance in Salix spp.

Premise:

The heritability of gene expression is critical in understanding heterosis and is dependent on allele-specific regulation by local and remote factors in the genome.  In this study, we used RNA-Seq to test whether variation in gene expression among F1 and F2 intraspecific Salix purpurea progeny is attributable to cis– and trans-regulatory divergence using two distinct tissue types: shoot tip and stem internode.  In addition, we explored sexually dimorphic patterns of gene expression inheritance and regulatory divergence among F1 individuals.

Altogether, our results offer insight into the inheritance of gene expression in S. purpurea as well as evidence of sexually dimorphic expression which may have contributed to the evolution of dioecy in Salix.

Get the Advanced Access article now:  Carlson CH, Choi Y, Chan AP, Serapiglia MJ, Town CD, Smart LB. 2017. Dominance and sexual dimorphism pervade the Salix purpurea L. transcriptome. Genome Biology and Evolution, evx174.

Impact of genotypic and environmental effects on variation in shrub willow biomass quality

Premise:

Yield improvement of woody bioenergy crops has been the major focus of breeding programs, but biomass quality is also important for conversion to biofuels. Using high-resolution thermogravimetric analysis, the composition of biomass samples from two shrub willow (Salix spp.) yield trial networks representing two distinct datasets were examined. Dataset 1 consisted of 12 yield trials containing 10 genotypes that mainly represented early cultivars from the US breeding program. Dataset 2 consisted of five trial locations containing 19 genotypes from later breeding efforts. The genetic variation in wood composition as well as the ability to modify composition via management, indicate that both new genetic resources and management can be used to optimize biofuel conversion efficiency.

Go to the article now: Fabio E.S., Volk T.A., Miller R.O., Serapiglia M.J., Kemanian A.R., Montes F., Kuzovkina Y.A., Kling G.J., Smart L.B. 2017. Contributions of environment and genotype to variation in shrub willow biomass composition. Industrial Crops and Products, 108: 149-161.

Paper update: mixed model evaluation of yield in shrub willow hybrids

fabio-et-al-2016-ind-crop-prod-96-57-70Premise:

“The use of mixed models has allowed us to analyze a large yet unbalanced dataset of shrub willow yields in order to characterize GEI. The main findings are incremental increases in yield were achieved through traditional breeding techniques, that interspecific triploid hybrids seem to express the largest yield gains, especially in warmer environments, and that the GEI allows for the identification of cultivars adapted to low and high-yielding environments. This is critical for deploying regionally adapted and high-yielding genotypes for stands expected to be productive over 20 years. As a result of this work we have identified a number of recently bred, high-yielding triploid hybrids from diverse pedigrees that will be advanced for commercialization.”

Go to the article now: Fabio, E.S, Kemanian, A.R., Montes, F., Miller, R.O., and Smart, L.B. (2016) A mixed model approach for evaluating yield improvements in interspecific hybrids of shrub willow, a dedicated bioenergy crop. Industrial Crops and Products, 96: 57-70.

Smart lab publishes paper using capacitance as a predictor of root dry wt. in shrub willow

Carlson and Smart 2016 APPS 4(8)1600031Premise:

Root biomass is an important trait often disregarded in woody perennial selection due to the challenge and expense of accurately and efficiently measuring large populations. In this study, we aim to develop a simple method that can predict root dry weight within a diverse shrub willow (Salix spp.) breeding population representing species hybrids and their parents using root electrical capacitance (REC). This work provides an efficient and nondestructive technique to indirectly quantify root biomass of genetically diverse shrub willow progeny, which has great promise for selection of genotypes with varying root biomass and for the accurate estimation of belowground carbon sequestration.

Go to the article now: Carlson, C.H. and Smart, L.B. (2016) Electrical capacitance as a predictor of root dry weight in shrub willow (Salix; Salicaceae) parents and progeny. Applications in Plant Sciences, 4(8):1600031.

New paper from Smart Lab on GxE in shrub willow

ericGCBClip

Premise:

Shrub willow is showing promise as a dedicated bioenergy crop in areas of North America. Early breeding efforts have demonstrated that its broad taxonomic diversity can be exploited for genetic improvement. However, a comprehensive evaluation of the yield potentials of currently available cultivars has not occurred in North America. We used the Additive Main effects and Multiplicative Interactions (AMMI) model to explore genotype by environment interactions for the identification of broad and specific adaptation. We confirmed ploidy level as a key genetic factor involved in yield improvements which will be important for developing cultivar recommendations and informing future breeding work.

Go to the article now: Fabio, E. S., Volk, T. A., Miller, R. O., Serapiglia, M. J., Gauch, H. G., Van Rees, K. C.J., Hangs, R. D., Amichev, B. Y., Kuzovkina, Y. A., Labrecque, M., Johnson, G. A., Ewy, R. G., Kling, G. J. and Smart, L. B. (2016), Genotype by environment interactions analysis of North American shrub willow yield trials confirms superior performance of triploid hybrids. GCB Bioenergy. Accepted Author Manuscript. doi:10.1111/gcbb.12344

 

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